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KMID : 1007520220310111451
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Food Science and Biotechnology
2022 Volume.31 No. 11 p.1451 ~ p.1462
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Mechanism of gastrointestinal adaptability and antioxidant function of infant-derived Lactobacillus plantarum BF_15 through genomics
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Wang Xinyu
Zhang Na
Li Dongyao
Wang Miaoshu
Li Chen
Tian Hongtao
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Abstract
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Lactobacillus plantarum is an essential probiotic in the human gastrointestinal tract. L. plantarum BF_15, a functional probiotic isolated from the feces of breast-fed infants, has been reported in many in vitro and in vivo studies with strong gastrointestinal adaptability and outstanding anti-oxidative activities. Therefore, the whole genome of L. plantarum BF_15 was sequenced. Several genes, encoding the gastrointestinal adaptability-related proteins, were identified, including genes related to gastrointestinal environment-induced stress resistance, adhesive performance, and ability to transport and metabolize resistant starch and oligosaccharides. Genes related to alleviating oxidative stress were also found. Further functional verification was carried out by RT-qPCR on the 10 and 12 key adhesion and antioxidant genes. Overall, this study might provide a critical basis for L. plantarum BF_15 as a potential candidate for probiotics.
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KEYWORD
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Lactobacillus plantarum, Genome, Gastrointestinal adaptability, Anti-oxidative capacity, Real-time quantitative PCR
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